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Published online 1 January 1982
Published in Agron J 74:59-62 (1982)
© 1982 American Society of Agronomy
677 S. Segoe Rd., Madison, WI 53711 USA
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Sodium Hypobromite Oxidation Method for Determination of Total Phosphorus in Plant Materials1

W. A. Dick and M. A. Tabatabai2

A simple, sensitive, precise, and rapid method for determination of total P in plant materials is described. The sample is heated with alkaline sodium hypobromite solution to oxidize the P compounds to phosphate, and the phosphate thus obtained is extracted with 1N H2SO4 and determined colorimetrically by a heteropoly blue color method. Comparison of results obtained by the hypobromite oxidation and HNO3-HCIO4 digestion procedures showed that the average total P values by the two methods for 20 plant samples and animal manures analyzed were 0.385 and 0.384%, respectively. In addition to being safe, digestion of plant samples with sodium hypobromite solution and colorimetric determination of the extracted P by the method of Murphy and Riley permits 100 analyses to be performed by a single operator in a normal working day.

Key Words: HNO3-HCIO4 • digestion • Heteropoly blue color • Plant analysis • Animal manures


1 Joint contribution: Journal Paper No. 61-81 of the Ohio Agric. Res. and Dev. Ctr., Wooster, OH 44601, and Journal Paper No. J-10209 of the Iowa Agric. and Home Econ. Exp. Stn., Ames, IA 50011 (Project 2431).

2 Assistant professor of agronomy, OARDC, Wooster, Ohio, and professor of agronomy, Iowa State Univ., Ames.

Received for publication April 8, 1981.





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Copyright © 1982 by the American Society of Agronomy.