Agronomy Journal Journal of Natural Resources and Life Sciences Education
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Published online 1 November 1981
Published in Agron J 73:996-998 (1981)
© 1981 American Society of Agronomy
677 S. Segoe Rd., Madison, WI 53711 USA
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Nitrogen Fixation Rates of Alfalfa and Red Clover Grown in Mixture with Grasses1

Lelia de Anda Craig, W. J. Wiebold and M. S. McIntosh2

Nitrogen contributions of legumes to grass-legume mixtures are estimated on the basis of legume monocultures and may need to be adjusted to reflect any effect of the companion grass. An experiment at two field locations was conducted to determine whether the N2-fixation rates of alfalfa (Medicago sativa L.) and red clover (trifolium pratense L.) were significantly affected by mixed culture with orchardgrass (Dactylis glomerata L.) or timothy (Phleum pratense L.). Legumes and grasses were grown in eight mono- or mixed cultures. Specific nodule activity (SNA) was estimated by acetylene reduction at first flower of legumes and 4 weeks after initial harvest. Grasses were analyzed for total N (TN). Legume dry weight per plant was reduced by association with grasses in only one determination at one location. Grass TN 4 weeks after initial harvest was in most instances increased significantly by the legume. Specific nodule activities of red clover grown alone and red clover grown with grasses were similar and did not change between determinations. The SNA of alfalfa grown with grass was significantly higher than that of alfalfa grown alone for the second determination at one location. The SNA of alfalfa in mixed culture increased between determinations; however, SNA of alfalfa monocultures did not change. It is concluded that grass plants are not detrimental to legume plants and may stimulate N2-fixation. Some transfer of N from legumes to grasses may occur when the two species are grown in mixed cultures.

Key Words: Medicago sativa L. • Trifolium pratense L. • Dactylis glomerata L. • Phleum pratense L. • Acetylene reduction • Total N • Specific module activity


1 Scientific Article No. A-2897, Contribution No. 5951 of the Maryland Agnc. Exp. Stn., Dep. of Agronomy.

2 Former graduate research assistant and assistant professors of agronomy. Univ. of Maryland, College Park, MD 20742.

Received for publication December 15, 1980.





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Copyright © 1981 by the American Society of Agronomy.