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Cold Hardening and Carbohydrate Composition of Meyer Zoysia¹

There are few reports on cold hardening in warmseason turfgrass species. The purpose of our study was to measure changes in cold hardiness and carbohydrate composition of Zoysia japonica Steud ‘Meyer’ turfgrass during fall and winter.

Both rhizome and stolon tissue developed comparable levels of cold hardiness as measured by an artificial freeze test. A significant (P <; 0.05) reduction in percentage survival of tissue occurred at freeze-test temperatures at or below –6.7 C on September 23 and Octobei 26; and –10.0, –8.9, and –12.8 C on November 30. January 10, and March 13, respectively. Total nonstructural carbohydrate (TNC) concentration of both rhizome and stolon tissue increased during September, remained relatively constant near 50% of dry weight until December, and was reduced to about 15% by March 15. Starch comprised the largest percentage of the carbohydrate fractions and closely followed TNC concentration. Total sugars increased slightly during fall, while reducing sugars remained nearly constant during the entire sampling period.

Key Words: Freeze test • Rhizomes • Stolons • Winter survival • Zoysia japonica Steud

² Graduate student and associate professor, respectively, Dep. of Hort., and associate professor, Dep. of Agron., Univ. of Missouri, Columbia, MO 65201.

Received for publication July 29, 1974.

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