Agronomy Journal Journal of Natural Resources and Life Sciences Education
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Published online 1 January 1973
Published in Agron J 65:109-112 (1973)
© 1973 American Society of Agronomy
677 S. Segoe Rd., Madison, WI 53711 USA
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Determination of Total Nitrogen in Plant Material1

D. W. Nelson and L. E. Sommers2

A simple and precise procedure for estimating total N in plant tissue is described; a modification is also described for analysis of samples containing high nitrate concentrations. Plant tissue samples are placed in pyrex Folin-Wu tubes and digested with a salt-catalyst-sulfuric acid mixture by heating the tubes in an aluminum block. Samples are digested at the boiling point of the mixture for 60 min after initial clearing of the digests. Analyses of diverse plant materials, containing from 0.006 to 0.3% NO3-N3 indicated that the proposed and standard AOAC procedures yielded essentially the same total N values. The coefficient of variation for the proposed methods varied from 0.87 to 1.10%. The tube digestion procedures described allows digestion of 60 plant samples simultaneously and thus greatly improve the efficiency of total N determinations.

Key Words: Tube digestion method


1 A contribution of the Agricultural Experiment Station, Purdue University, Lafayette, Ind. 47907. Purdue Journal Paper No. 4742. Laboratory assistance by Mrs. Paz Koh is appreciated.

2 Assistant Professors of Agronomy.

Received for publication May 19, 1972.





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Copyright © 1973 by the American Society of Agronomy.