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Depletion of Nitrate Reductase Activity in Response to Soil Leaching¹

Nitrate leaches readily from sandy soils. To test the sensitivity of nitrate reductase (a substrate-inducible enzyme) as an indicator of nitrate leaching and restoration, low, normal, and high N treatments were imposed on young tobacco (Nicotiana tabacum L.) plants grown on a Durham sandy loam soil (Typic hapludult). Subsequent treatments imposed were soil leaching with and without a later application of replacement fertilizer. Plants were sampled at frequent intervals to determine the rapidity of changes in tissue nitrate concentration and nitrate reductase activity. Tissue nitrate concentration decreased within 24 hours after leaching in all N treatments in 1968. Nitrate reductase activity was decreased in the low and normal N treatments. The addition of replacement fertilizer resulted in increased nitrate reductase activity in the low and normal N treatments within 24 hours.

Key Words: Enzyme • Tobacco

² Assistant Professor, Crop Science Department, and Professor, Soil Science Department, North Carolina State University, Raleigh, N. C. 27607.

Received for publication March 20, 1972.