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Leaf Analysis as a Selection Index for Soybean Seed Oil and Protein¹

Leaf and seed samples were obtained from 46 lines of soybeans in four maturity groups in 1969 to determine the correlation between selected elements in the leaf with oil and protein of the seed. Leaf samples were chemically analyzed by standard procedures for their N, P, K, Ca, Mg, B, Cu, Ye, Mn, Zn, and Na content. The N content of seed was determined by standard Kjeldahl methods and their oil content was measured with an NMR Process Analyzer. Simple correlations were computed between leaf components, maturity date, oil, and protein.

Significant correlations were found between leaf components and maturity date, and leaf components and oil or protein content of the seed. Partial correlation coefficients were determined after accounting for variability due to maturity date. A statistically significant negative association between leaf Zn and protein content of the seed was found. It was concluded that selection of germ plasm based on correlations between leaf components and oil or protein would also be selection of germ plasm based on correlation with date of maturity. After accounting for variability due to date of maturity, chemical composition of the soybean leaf was a relatively poor predictor of future oil or protein content of the seed.

Key Words: Glycine max (L.) Merr. • Maturity group

² Assistant Professor of Plant Genetics and Associate Professor of Biometry and Soil Fertility, Department of Agronomy, University of Illinios at Urbana-Champaign. The authors gratefully acknowledge the assistance given by the personnel of the USDA Regional Soybean Laboratory, Urbana, and the Department of Agronomy Emission Spectrographic Laboratory for the leaf and seed analysis.

Received for publication February 26, 1970.