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In-the-furrow Application of Root-knot Larvae in Field Plots¹

A method of inoculating a small field plot with Northern root-knot nematode, Meloidogyne hapla Chitwood, is described. An average of 11,353 nematode larvae that had been collected from tomato roots under a mist system in the greenhouse were distributed per 30.48 cm (foot) of row during planting. The larvae were dispensed in the furrow over peanut seeds by gravity flow through a glucose intravenous injection apparatus mounted on a garden planter.

Differences in plant growth and pod yields in the inoculated and uninoculated plots were not apparent the 1st year. Visual differences in growth and statistically significant differences in pod yield and plant height, but not in stand, occurred the 2nd year after inoculation. Pod yields and plant heights were reduced 66 and 29%, respectively, in the nematode infested plots.

Key Words: Peanut • Tomato roots • Mist system • Garden planter • Austrian Winter field peas

² Research Geneticist, Crops Research Division, ARS, USDA, Stillwater, Okla., and Associate Professor of Agronomy, Okla. Agr. Exp. Sta., Stillwater, Okla. 74074

Received for publication November 10, 1969.