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Published online 1 January 1970
Published in Agron J 62:36-39 (1970)
© 1970 American Society of Agronomy
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Adaptation of Gas-Liquid Chromatography to Analysis of Certain Sugars of Cotton Leaves1

Wayne E. Sabbe and George W. Cathey2

A procedure involving gas-liquid chromatography (GLC) of certain sugars in cotton leaves was studied. The use of hexamethyl-disilazane (HMDS) and trimethylchlorosilane (TMS) for silylation of fructose, glucose, and sucrose gave recovery percentages ranging from 71 to 105%. The substitution of trifluoracetic acid (TFA) for TMS gave recovery percentages of 91 to 109%. The use of TFA also improves the ease of sample preparation since absolute exclusion of water was not necessary. Details of sample preparation and operating parameters of GLC are given.

Key Words: Gas chromatography • Aqueous extraction • Trimethylsilyl derivatives

1 Joint contribution of Crops Research Division, Agricultural Research Service, U.S. Department of Agriculture, and the Arkansas Agricultural Experiment Station, Fayetteville 72701. Published with approval of the Director of the Arkansas Agricultural Experiment Station.

2 Assistant Agronomist, University of Arkansas, and Plant Physiologist, Crops Research Division, ARS, USDA, Delta Branch Experiment Station, Stoneville, Miss, (formerly Research Technician, CRD, ARS, USDA, University of Arkansas, Fayetteville).

Received for publication March 3, 1969.




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Copyright © 1970 by the American Society of Agronomy.