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Storage of Soybean Pollen for Artificial Crossing¹

Soybean pollen desiccated rapidly when removed from the anthers and could not be stored for artificial crossing in any humidity at 26 C. Pollen could be stored in the flowers for at least 8 hr in constant humidities ranging from 0 to 80% relative humidity (RH), but viability was lost in humidities above 80% RH. Pollen viability was drastically reduced when whole flowers were stored in alternating humidities.

For artificial crossing in warm and humid environments, pollen in whole flowers can be stored at least 8 hr from gathering time to actual crossing at 26 C and 70% RH.

Key Words: Constant and alternating humidities • Desiccation

² Research Agronomist now located at Mesa, Arizona, P. O. Box 858, 85201. The author gratefully acknowledges the suggestions of W. F. Lehman of the University of California, El Centro, Calif.

Received for publication June 13, 1969.