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Mountain soils subjected to excessive irrigation and high water tables accumulate organic matter in sod mats. Meadow soils often contain two or three times more N than equal areas of adjacent dry soils. Symbiotic nitrogen fixation by legumes is one possible source of N for sod mat formation. Fixation of N2 by free-living organisms has also been postulated and was verified by the laboratory studies reported here. Nitrogen fixation by sod mats containing no legumes was determined at 18C in atmospheres containing N215 Both photosynthetic and nonphotosynthetic organisms appeared to fix N2. In 10 days, mats in the dark fixed from 0.76 to 1.90 kg N2 ha–1 and illuminated mats fixed from 3.72 to 6.86 kg N ha–1.
Key Words: Mountain meadows Nitrogen-15
2 Research Soil Scientists, USDA, Fort Collins, Colo. 80521 and Mandna, N. D., respectively.
Received for publication November 29, 1968.
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