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Two sources of beta radiation, carbon-14 (C14) and promethium-147 (Pm147), were investigated for determination of leaf water content of three plant species over a range of water stress conditions. The plant species used were soybean (Glycine max L.), sorghum (Sorghum vulgare L.), and barley (Hordeum vulgare L.). Results obtained with both sources gave high correlations of relative turgidity with attenuated radiation. However, for relative turgidities above 60% in the plants studied, higher correlation coefficients were obtained using the C14 source. Results indicated that the C14 method appeared to be more sensitive to small changes in relative turgidity than was the Pm147 method. Leaf disc sections taken from sorghum and barley leaves gave more uniform response to water stress changes than did whole leaves.
Key Words: relative turgidity C14 Pm147
2 Formerly student South Dakota State University, now graduate assistant University of California, Davis 95616, and Associate Professor of Agronomy, South Dakota State University, Brookings 57006, respectively.
Received for publication November 21, 1967.
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