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Published online 1 July 1968
Published in Agron J 60:333-336 (1968)
© 1968 American Society of Agronomy
677 S. Segoe Rd., Madison, WI 53711 USA
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Two Beta Sources Compared for Evaluating Water Status of Plants1

D. E. Rolston and M. L. Horton2

Two sources of beta radiation, carbon-14 (C14) and promethium-147 (Pm147), were investigated for determination of leaf water content of three plant species over a range of water stress conditions. The plant species used were soybean (Glycine max L.), sorghum (Sorghum vulgare L.), and barley (Hordeum vulgare L.). Results obtained with both sources gave high correlations of relative turgidity with attenuated radiation. However, for relative turgidities above 60% in the plants studied, higher correlation coefficients were obtained using the C14 source. Results indicated that the C14 method appeared to be more sensitive to small changes in relative turgidity than was the Pm147 method. Leaf disc sections taken from sorghum and barley leaves gave more uniform response to water stress changes than did whole leaves.

Key Words: relative turgidity • C14 • Pm147


1 Contribution from the Department of Agronomy, Agr. Exp. Sta., South Dakota State University, Brookings. Approved for publication by the Director of the South Dakota Agr. Exp. Sta. as Journal Series No. 797. Presented before Div. A-3, American Society of Agronomy, Nov. 3, 1965, at Columbus, Ohio.

2 Formerly student South Dakota State University, now graduate assistant University of California, Davis 95616, and Associate Professor of Agronomy, South Dakota State University, Brookings 57006, respectively.

Received for publication November 21, 1967.





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